By Matthew D. Weitzman, R. Michael Linden (auth.), Richard O. Snyder, Philippe Moullier (eds.)
Today, growth in rAAV-mediated gene move is so strong that long term, effective, and regulatable transgene expression is reproducibly completed in huge animal types. The complexity of gene move brokers within the context in their medical use calls for investigators from a wide selection of backgrounds to have an knowing — or no less than an appreciation of — the regulatory setting and constraints that have an effect on vector layout, production, pre-clinical trying out, and scientific use, with an emphasis on sufferer safeguard. In Adeno-Associated Virus: equipment and Protocols, specialists from the us and Europe have contributed present wisdom of this multi-dimensional box in terms of the biology of AAV, rAAV vector layout, vector production and product checking out, functionality of rAAV vectors in significant organs, rAAV-related immunological matters, layout of animal and scientific reports, and scientific event. Written within the profitable Methods in Molecular Biology™ sequence layout, chapters comprise introductions to their respective subject matters, lists of the required fabrics and reagents, step by step, without problems reproducible protocols, and notes on troubleshooting and fending off identified pitfalls.
Authoritative and available, Adeno-Associated Virus: tools and Protocols presents an entire and accomplished realizing of this multi-disciplinary and quickly progressing field.
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Extra info for Adeno-Associated Virus: Methods and Protocols
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It is likely that the dynamic relationship between understanding basic biology and developing vectors will continue, and advances in each area will propel the applications that are possible for gene therapy. Acknowledgments This article is an overview of AAV basic biology and is not an exhaustive review of the topic. We apologize for primary work not covered here either from lack of space or inadvertent oversight. D. M. Linden and a Pioneer Developmental Chair from the Salk Institute. AAV work in the Linden laboratory was supported by NIH grants GM07390, 1GM071023, and GM075019, and by King’s College London School of Medicine.
Adeno-Associated Virus: Methods and Protocols by Matthew D. Weitzman, R. Michael Linden (auth.), Richard O. Snyder, Philippe Moullier (eds.)