Adeno-Associated Virus: Methods and Protocols - download pdf or read online

By Matthew D. Weitzman, R. Michael Linden (auth.), Richard O. Snyder, Philippe Moullier (eds.)

ISBN-10: 1617793698

ISBN-13: 9781617793691

ISBN-10: 1617793701

ISBN-13: 9781617793707

Today, growth in rAAV-mediated gene move is so strong that long term, effective, and regulatable transgene expression is reproducibly completed in huge animal types. The complexity of gene move brokers within the context in their medical use calls for investigators from a wide selection of backgrounds to have an knowing — or no less than an appreciation of — the regulatory setting and constraints that have an effect on vector layout, production, pre-clinical trying out, and scientific use, with an emphasis on sufferer safeguard. In Adeno-Associated Virus: equipment and Protocols, specialists from the us and Europe have contributed present wisdom of this multi-dimensional box in terms of the biology of AAV, rAAV vector layout, vector production and product checking out, functionality of rAAV vectors in significant organs, rAAV-related immunological matters, layout of animal and scientific reports, and scientific event. Written within the profitable Methods in Molecular Biology™ sequence layout, chapters comprise introductions to their respective subject matters, lists of the required fabrics and reagents, step by step, without problems reproducible protocols, and notes on troubleshooting and fending off identified pitfalls.

Authoritative and available, Adeno-Associated Virus: tools and Protocols presents an entire and accomplished realizing of this multi-disciplinary and quickly progressing field.

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Chang, L. , and Shenk, T. (1991) Transcriptional repression by YY1, a human GLI-Kruppel-related protein, and relief of repression by adenovirus E1A protein, Cell 67, 377–388. 101. Chang, L. , and Shenk, T. (1989) Adeno-associated virus P5 promoter contains an adenovirus E1A-inducible element and a binding site for the major late transcription factor, J Virol 63, 3479–3488. 102. Weitzman, M. , Fisher, K. , and Wilson, J. M. (1996) Recruitment of wild-type and recombinant adeno-associated virus into adenovirus replication centers, J Virol 70, 1845–1854.

94. 95. 96. 97. 98. 99. 100. D. M. Linden virus: properties of deletion mutants constructed in vitro and evidence for an adenoassociated virus replication function, J Virol 51, 611–619. , and Muzyczka, N. (2008) Complete in vitro reconstitution of adenoassociated virus DNA replication requires the minichromosome maintenance complex proteins, J Virol 82, 1458–1464. Ni, T. , McCarty, D. , and Muzyczka, N. (1994) In vitro replication of adeno-associated virus DNA, J Virol 68, 1128–1138. Ward, P. (2006) Replication of adeno-associated virus DNA, in Parvoviruses (Jonathan R.

It is likely that the dynamic relationship between understanding basic biology and developing vectors will continue, and advances in each area will propel the applications that are possible for gene therapy. Acknowledgments This article is an overview of AAV basic biology and is not an exhaustive review of the topic. We apologize for primary work not covered here either from lack of space or inadvertent oversight. D. M. Linden and a Pioneer Developmental Chair from the Salk Institute. AAV work in the Linden laboratory was supported by NIH grants GM07390, 1GM071023, and GM075019, and by King’s College London School of Medicine.

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Adeno-Associated Virus: Methods and Protocols by Matthew D. Weitzman, R. Michael Linden (auth.), Richard O. Snyder, Philippe Moullier (eds.)


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